The innate immune response to bacterial infections
Sylvia Knapp’s research concentrates on the innate immune response to bacterial infections in general, focusing specifically on the comprehensive repertoire of macrophage functions in health, development and disease. Her investigations concentrate on the initiation and resolution of clinically relevant infections and on the role of endogenous danger molecules and their interactions with host structures and pathways. Her latest research is directed towards the interplay of immune cells in regulating tissue homeostasis in health and disease.
Innate immunity is the evolutionary conserved arm of the immune system and instrumental in maintaining tissue homeostasis while at the same time providing the tools to immediately sense and respond to danger such as invading pathogens, metabolic stress or injuries. This wide and diverse range of tasks requires an incredible plasticity and flexibility of the players involved, with tissue resident macrophages being essential in orchestrating many of these responses.
Using clinically relevant infection, injury and metabolic stress models, research in the Knapp-Lab is focusing on how host factors can influence the balance of innate responses in steady state and upon danger.
Dysregulation in the balance of innate immune mechanisms can profoundly alter the susceptibility to bacterial and viral infections, and predisposes to chronic diseases. A prime example of dysregulated immune homeostasis occurs following viral lung infections or acute lung injury, which are the medically most important risk factors for secondary bacterial pneumonia. We focus on how host factors impact on pulmonary immune homeostasis in an attempt to identify immune-modulatory mechanisms that can be used to maintain tissue homeostasis and thus prevent secondary infections.
Using systems approaches, the Knapp-Lab aims to unravel temporal response patterns in a given tissue upon infection, to understand the long-term effects of disrupted homeostasis herein. In a second line of research we investigate how perturbed homeostasis in the course of inflammatory or inherited disorders affects the functionality of immune effector cells, thusly affecting outcome from common infectious diseases such as Gram-negative sepsis or pneumococcal pneumonia.
A central question addressed by the Knapp-Lab pertains to the huge functional repertoire of tissue resident macrophages. As such we study how alveolar macrophages, the tissue resident macrophage subset in lungs, exert their pleiotropic functions upon danger and the forces that maintain these cells in a de-activated phenotype at homeostasis. We have identified several players that importantly shape alveolar macrophage functionality and demonstrated their relevance in pneumonia models.
Sylvia Knapp, MD, PhD, is Professor of Infection Biology at the Medical University of Vienna. Sylvia studied Medicine in Vienna and Berlin, is a board-certified internist and obtained her PhD at the University of Amsterdam. In 2006, she joined CeMM as a Principal Investigator and until recently, she continued her clinical duties while also running her own lab. Sylvia’s research focuses on the innate immune response to infections in general, focusing specifically on the comprehensive repertoire of macrophage functions in health, development, and disease. Her group discovered the molecular mechanisms linking hemolysis and susceptibility to infections. Her latest research is directed towards the interplay of immune cells regulating lung tissue homeostasis in health and disease. Sylvia is highly committed to bridging academic medicine and basic science. She is a member of the Academia.Net circle of excellent female scientists and was elected corresponding member of the Austrian Academy of Sciences in 2014. In 2018, Sylvia was appointed to the University Board of the Medical University of Graz and elected vice president of the Ludwig Boltzmann Society.
Cohen M, Giladi A, Gorki AD, Solodkin DG, Zada M, Hladik A, Miklosi A, Salame TM, Halpern KB, David E, Itzkovitz S, Harkany T, Knapp S, Amit I. Lung Single-Cell Signaling Interaction Map Reveals Basophil Role in Macrophage Imprinting. Cell. 2018 Nov 1;175(4):1031-1044.e18.(abstract)
Saluzzo S, Gorki AD, Rana BMJ, Martins R, Scanlon S, Starkl P, Lakovits K, Hladik A, Korosec A, Sharif O, Warszawska JM, Jolin H, Mesteri I, McKenzie ANJ, Knapp S. First-Breath-Induced Type 2 Pathways Shape the Lung Immune Environment. Cell Rep. 2017 Feb 21;18(8):1893-1905. (abstract)
Martins R, Maier J, Gorki AD, Huber KV, Sharif O, Starkl P, Saluzzo S, Quattrone F, Gawish R, Lakovits K, Aichinger MC, Radic-Sarikas B, Lardeau CH, Hladik A, Korosec A, Brown M, Vaahtomeri K, Duggan M, Kerjaschki D, Esterbauer H, Colinge J, Eisenbarth SC, Decker T, Bennett KL, Kubicek S, Sixt M, Superti-Furga G, Knapp S. Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. Nat Immunol. 2016 Dec;17(12):1361-1372. (abstract)
Maier BB, Hladik A, Lakovits K, Korosec A, Martins R, Kral JB, Mesteri I, Strobl B, Müller M, Kalinke U, Merad M, Knapp S. Type I interferon promotes alveolar epithelial type II cell survival during pulmonary Streptococcus pneumoniae infection and sterile lung injury in mice. Eur J Immunol. 2016 Sep;46(9):2175-86. (abstract)
Castiglia V, Piersigilli A, Ebner F, Janos M, Goldmann O, Damböck U, Kröger A, Weiss S, Knapp S, Jamieson AM, Kirschning C, Kalinke U, Strobl B, Müller M, Stoiber D, Lienenklaus S, Kovarik P. Type I Interferon Signaling Prevents IL-1β-Driven Lethal Systemic Hyperinflammation during Invasive Bacterial Infection of Soft Tissue. Cell Host Microbe. 2016 Mar 9;19(3):375-87. (abstract)
Sharif O, Gawish R, Warszawska JM, Martins R, Lakovits K, Hladik A, Doninger B, Brunner J, Korosec A, Schwarzenbacher RE, Berg T, Kralovics R, Colinge J, Mesteri I, Gilfillan S, Salmaggi A, Verschoor A, Colonna M, Knapp S. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathog. 2014 Jun 12;10(6):e1004167. (abstract)
Warszawska JM, Gawish R, Sharif O, Sigel S, Doninger B, Lakovits K, Mesteri I, Nairz M, Boon L, Spiel A, Fuhrmann V, Strobl B, Müller M, Schenk P, Weiss G, Knapp S. Lipocalin 2 deactivates macrophages and worsens pneumococcal pneumonia outcomes. J Clin Invest. 2013 Aug;123(8):3363-72. (abstract)
Matt U, Sharif O, Martins R, Furtner T, Langeberg L, Gawish R, Elbau I, Zivkovic A, Lakovits K, Oskolkova O, Doninger B, Vychytil A, Perkmann T, Schabbauer G, Binder CJ, Bochkov VN, Scott JD, Knapp S. WAVE1 mediates suppression of phagocytosis by phospholipid-derived DAMPs. J Clin Invest. 2013 Jul;123(7):3014-24. (abstract)
Baumann CL, Aspalter IM, Sharif O, Pichlmair A, Blüml S, Grebien F, Bruckner M, Pasierbek P, Aumayr K, Planyavsky M, Bennett KL, Colinge J, Knapp S, Superti-Furga G. CD14 is a coreceptor of Toll-like receptors 7 and 9. J Exp Med. 2010 Nov 22;207(12):2689-701. (abstract)